What Is A Urine Culture

Contents

What You Need to Know About Urine Cultures

Your doctor informs you that an aurine culture will be performed. It’s a test to see if you have any germs or bacteria in your pee that might cause an infection of the urinary system (UTI). The urinary system is made up of the kidneys, the bladder, and the tubes that transport your urine (ureters and the urethra). In most cases, an infection begins in the bladder or urethra (the tube your pee comes out of). However, it has the potential to impact any aspect of the system. Infections can cause a burning sensation when you pee, which can be quite uncomfortable.

Having a fever and stomach ache may indicate a more serious infection, so seek medical attention immediately.

What Do I Do for a Urine Culture?

You have to pee in a cup. It appears to be straightforward, and it is. Simply ensure that you get a “clean” urine sample to ensure that any germs identified in it are from an illness in your urinary system and not from another source, such as your skin, before proceeding. Here’s how to go about it:

  1. Wipe the area surrounding where you pee with the cleaning pad that was provided to you. Wash your hands. If you’re a woman, spread the outer lips of your vagina and clean it from front to back from front to back from front to back. Men should clean the tip of their penis first, then pee a small amount in the toilet and quit rubbing their penis. Don’t pee in the cup until you’re ready. Then, place roughly 1 or 2 ounces of the mixture in a cup. Make certain that the container does not come into contact with your skin. Complete your peeing in the toilet. A pee capture in the middle of the stream is referred to as a “midstream” urine catch. Wash your hands once more

Some people may require the collection of their sample with the use of a catheter, which is a tiny tube that is inserted into your urethra and into your bladder. This is accomplished with the assistance of a health-care professional. The sample is put in a new, clean container after being cleaned.

What Happens Next?

Your sample is sent to a laboratory for analysis. Some of your urine is collected in a petri plate and kept at room temperature. Any bacteria or yeast present in the sample will reproduce and increase over the course of the following several days. The germs will be examined under a microscope by a lab technician. The different varieties are distinguished by their size, shape, and color. The lab technician will keep track of how many are growing. If it is a real illness, one species of bacteria will generally dominate the situation.

If the culture does not contain any hazardous microorganisms, it is referred to be “negative.” If there are harmful microorganisms developing, this is considered “positive.” E-coli, a kind of bacteria that lives in your intestines, is the most common cause of urinary tract infections.

When Will I Get My Results?

Your doctor’s office will contact you within one to three days. They’ll go through the results with you when they’re done. If you have an illness, you will almost certainly be prescribed antibiotics. If this is the case, make certain you complete the total amount specified. The majority of the time, the infection subsides. However, it is possible that it will return, especially if you are a sexually active woman. In young women, sexual contact increases the likelihood of contracting an infection.

Men over the age of 50 who have an enlarged prostate are at greater risk. It is important that you take your medication in the manner prescribed by your doctor. An infection that begins in the bladder or urethra has the potential to move to the kidneys and cause harm.

Urine Culture

A urine culture is a test that is used to identify microorganisms (such as bacteria) in the urine that may be responsible for the ailment. It is possible for bacteria to enter the urinary tract and produce an infection of the urinary tract (UTI). It is necessary to add a sample of urine to a chemical that encourages the development of germs. If no germs appear to be growing, the culture is considered negative. If germs proliferate, the culture is said to be positive. It is possible to determine the kind of germ by using a microscope or chemical testing.

This may be due in part to the fact that the female urethra is shorter and closer to theanus than the male.

Men also have an antibacterial substance in their prostate gland that helps to reduce their chance of developing prostate cancer.

Why It Is Done

When a urine culture is performed, it may be determined whether symptoms such as discomfort or burning when peeing are caused by a urinary tract infection (UTI). The test can also be used to detect the origin of a UTI, assist in determining the most effective therapy for a UTI, and assess whether the treatment has been effective.

How To Prepare

You will need to obtain a urine sample for testing purposes. You will need to consume enough water and refrain from peeing in order to be able to provide a urine sample. Because bacterial counts will be greater in the first pee of the day, the first urine of the day is the best. It is best not to urinate just before taking this test.

How It Is Done

It is possible that you will be requested to collect a clean-catch midstream urine sample for testing purposes.

Clean-catch midstream urine collection

This approach aids in protecting the urine sample from germs that are generally located on the penis or vaginal area of the subject.

  1. Before collecting the pee, wash your hands well. If the collecting cup has a lid, carefully remove it from the cup. Place the lid on the table with the inside surface facing up. Don’t let your fingertips come into direct contact with the interior of the cup
  2. Make sure the region surrounding your genitals is clean.
  • Men should retract their foreskin if they have one, and wipe the head of their penis with medicated towelettes or swabs
  • Women should spread open the vaginal folds of skin with one hand
  • And men should retract their foreskin if they have one. Then she can use her other hand to wipe the region surrounding the urethra with medicated towelettes or swabs, which will relieve the pain. Ideally, she should clean the region from front to back in order to prevent bacteria from the anus from spreading over the urethra.
  1. Begin urinating into a toilet or urinal as soon as possible. While urinating, a lady should keep her vaginal folds apart
  2. Once the urine has flowed for several seconds, she should insert the collecting cup into the urine stream. Collect approximately 2 fl oz (59 mL) of urine without interfering with the flow of the urine. Move the cup out of the way of the urine flow. Do not allow the rim of the cup to come into contact with your genital area. It is not acceptable to have toilet paper, pubic hair, stool (feces), menstrual blood, or anything else in the urine specimen. Complete your urination into the toilet or urinal. Replace the lid on the cup with care and tighten it down. Then you should return the cup to the laboratory. If you collect the urine at home and are unable to make it to the lab within an hour, place it in the refrigerator.

Other collection methods

In order to obtain a urine sample, a health practitioner must insert a urinary catheter into the bladder of the patient. This procedure is often used to collect urine from a patient in the hospital who is severely unwell or who is unable to give a clean-catch sample using the traditional method. The use of a catheter to collect a urine sample lowers the likelihood of microorganisms from the skin or vaginal region becoming contaminated with the urine sample. It is possible to collect a urine sample from a tiny toddler or infant by utilizing a specific plastic bag that has been taped shut around the entrance (a U bag).

The bag is then carefully removed from the body.

(This procedure is referred to as a suprapubic tap.)

How long the test takes

It will only take a few minutes to complete the exam.

How It Feels

In most cases, there is no discomfort or suffering associated with this test.

Risks

There are no known dangers associated with undergoing this test.

Results

The findings of a urine culture are normally available in one to three days. Some bacteria, on the other hand, take longer to proliferate in the culture. As a result, it is possible that results will not be accessible for many days.

Urine culture

Normal: No bacteria or other germs (such asfungi) grow in the culture. The culture result isnegative.
Abnormal: Organisms (usually bacteria) grow in the culture. The culture result ispositive.

Credits

As of September 23, 2020, the information is current. Author:Healthwise StaffMedical Review: E. Gregory Thompson, MD – Internal MedicineE. Gregory Thompson, MD – Internal Medicine Dr. Adam Husney is a Family Medicine specialist. Dr. Elizabeth T. Russo specializes in Internal Medicine. As of September 23, 2020, the information is current. Written by a member of the Healthwise teamMedical Review: E. Gregory Thompson, MD, Internal Medicine Dr. Adam Husney is a Family Medicine specialist. Dr. Elizabeth T.

Urine Culture: How the Test Works

Overview When you have a urine culture, it can be used to identify bacteria in your urine. When a urinary tract infection occurs, this test can detect and identify the microorganisms that are causing it (UTI). The urethra is a passageway via which bacteria, which are primarily responsible for UTIs, can enter the urinary system. These bacteria can multiply fast in the environment of your urinary system, eventually resulting in an infection of the tract. More information may be found at: Do you want to discover everything there is to know about urinary system infection?

Females are more likely than males to get urinary tract infections.

As a result, germs from the intestines have a lot easier time making their way into the urinary system.

The following are the most frequent symptoms of a UTI:

  • Physical symptoms include: back and stomach pain
  • Fever
  • A strong need to pee often
  • Trouble passing urine through your urine stream
  • And urinary tract infection (UTI).

If you have a urinary tract infection (UTI), your urine may seem hazy or even turn pinkish or coral in color if there is blood present in it. Despite the fact that you may have a continual need to pee, you may be unable to pass more than a little volume of urine from your bladder due to bladder obstruction. Shaking, chills, and vomiting are common symptoms of an illness that is progressing to a dangerous stage. Several alternative methods are available for collecting urine for the purpose of doing a urine culture.

When you pee, you should collect your urine in a cup as you go.

Urinary collection bag

The urine of someone who has a urinary tract infection (UTI) may be murky or can turn pinkish or coral in color if there is blood present. In spite of the fact that you may have an insatiable need to pee, you may be unable to pass more than a little volume of urine from your bladder. Shaking, chills, and vomiting are all possible symptoms of an illness that is getting more serious.

Several alternative methods are available for collecting urine for a urine culture. It is the midstreamclean-catch approach that is the most commonly used for urine collection. The urine is collected in a cup while you pee in order to do this.

Catheter

In some circumstances, a catheter is required by a healthcare professional in order to collect a urine sample. A thin rubber tube is inserted through the urethra and into the bladder in order to do this. Once the catheter is in place, the healthcare professional can take a sample of the patient’s blood. Even if you already have a urinary catheter in place, your healthcare professional may be able to obtain a sample by closing the drainage end of your catheter before it reaches the drain bag. Once urine has been collected in the clamped tube, your healthcare professional will use a syringe to retrieve a sample of urine from the tube.

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Suprapubic aspiration

The use of a needle to extract a urine sample from your bladder may be required in some rare situations by your doctor. If prior attempts to acquire an uncontaminated sample have been failed, this method, known as a suprapubic aspiration, is utilized to collect the sample. It is possible that your obstetrician will ask you to do a urine culture at various intervals throughout your prenatal care as a preventive step if you are pregnant. If you suffer a urinary tract infection (UTI) during your pregnancy, it is critical that you get treatment as soon as possible.

  • Untreated urinary tract infections (UTIs) might result in preterm childbirth or poor labor outcomes.
  • Preparing for and carrying out a urine collection pose no dangers to the individual conducting it.
  • If your doctor demands a urine sample, you may experience some pressure and discomfort.
  • A catheter can occasionally cause a hole to form in your urethra or bladder.
  • Pre-testing preparations should include informing your doctor if you are currently taking or have recently taken any prescriptions, or if you are taking any over-the-counter vitamins, medicines, or supplements.
  • Aside from cleaning your hands and your genitals before the clean-catch collection, there is no need to prepare for a urine culture prior to the collection.
  • If you have any questions or concerns regarding the test, the dangers involved, or the results, you should discuss them with your doctor immediately.

Following that, the material is inspected under a microscope.

If just a small number of germs or organisms are detected, you will obtain a negative test result.

The results of a urine culture are usually available in two to three days.

It is possible that your sample contains more than one type of bacteria, or that it contains just a very little amount of bacteria.

It is possible that you will be required to retake the test.

coli bacteria, which may be found in your feces.

Some cases of urinary tract infections are caused by Candida, which is yeast that has the ability to overgrow and create an infection.

Most of the time, antibiotics are used to treat a urinary tract infection.

If you continue to get numerous UTIs, you may need to be tested to see whether you are more susceptible to them.

Every chance you have to attempt to flush out some of the germs can aid your body’s recovery by allowing it to return to normal faster.

Consider them to be ammo for your white blood cells, which will be used to battle the illness.

Once upon a time, it was believed that drinking unsweetened cranberry juice would help to expel harmful germs from the urinary system.

If you believe you have a urinary tract infection, it’s most likely because you’re experiencing unpleasant symptoms.

Wearing loose-fitting, cotton underwear and cleaning your clothes on a regular basis will help prevent the infection from returning.

However, if you suspect you have one, get medical attention immediately.

If you are experiencing pain in your low back or side below your ribs, as well as feeling shaky and weak, you should not dismiss these signs. If you experience any of these symptoms, you should consult your doctor.

Urine culture: MedlinePlus Medical Encyclopedia

A urine culture is a laboratory test that is used to determine if a urine sample contains bacteria or other pathogens. It may be used to screen for a urinary tract infection in both adults and children, according to the manufacturer. A clean catch urine sample will be taken in most cases, either at your health-care provider’s office or at your residence. To collect the urine, you will need to utilize a specific kit. An alternative method of collecting a urine sample is to put a thin rubber tube (catheter) into the urethra and into the bladder.

  1. The urine is drained into a sterile container, and the catheter is then pulled out of the bladder.
  2. The urine is sent to a laboratory for testing to detect whether or not bacteria or yeast are present in the urine sample.
  3. Try to collect the sample after your pee has been sitting in your bladder for 2 to 3 hours, if at all feasible.
  4. The urethra is numbed with the use of a specific gel.
  5. After you have been treated for an illness, you may be required to have a urine culture taken.
  6. “Normal growth” is a perfectly normal outcome.
  7. In certain cases, the normal value ranges may change somewhat between various laboratories.

Speak with your doctor about the significance of the specific test findings that you received.

Other tests may be performed to assist your physician in determining which bacteria or yeast is causing the illness and which antibiotics will be most effective in treating it.

If your provider uses a catheter, there is a very small chance that a hole (perforation) will develop in the urethra or bladder.

urine for culture and sensitivity testing Cooper KL, Badalato GM, Rutman MP; Badalato GM, Rutman MP.

The following chapter is from the 12th edition of Campbell-Walsh-Wein Urology: Chap 55.

Campbell-Walsh-Wein Urology, 12th edition, Philadelphia, PA: Elsevier; 2021:chap 55.

Nicolle LE, Drekonja D.

Philadelphia, PA: Elsevier; 2020: chap 268.

Vorvick, MD, Clinical Associate Professor, Department of Family Medicine, University of Washington Medicine, School of Medicine, University of Washington, Seattle, WA, provided the most recent update.

In addition, David Zieve, MD, MHA, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial staff examined the manuscript for accuracy.

What is a Urine Culture Sample?

Males must prepare for this test by wiping the top of their penis with a sterile wipe before taking it. Females should cleanse and rinse their vaginal region with a sterile wipe or soapy water to prevent infection. It is possible to complete this procedure in the restroom of your doctor’s office. It is necessary to initially let a tiny volume of pee to fall into the toilet before collecting an accurate sample. After that, place around three to six ounces in a sterile container. After enough pee has been collected, the container can be withdrawn from the urine stream.

  1. The use of a catheter to collect your urine may increase your chance of developing a urinary tract infection.
  2. The urine sample can be submitted to a lab for a more thorough review, or it can be analyzed in the doctor’s office if the doctor so desires.
  3. At the conclusion of that period, if there is no evidence of bacterial growth, the culture is negative.
  4. In order to treat the infection, antibiotics are selected based on the bacteria that is present.

Urine Test: Routine Culture (for Parents)

Urine cultures are routinely performed to determine the number of germs (microorganisms such as bacteria) present in the urine sample. The urine sample will be kept in circumstances that allow germs to proliferate after it has been collected by a technician. If there is no illness, it is normal for there to be just a minimal number of bacteria in the urine. The technician will use a microscope or chemical tests to discover which types of germs are present in the culture if a high number of germs are present.

Why It’s Done

In order to identify a urinary tract infection (UTI) and determine the kind of bacteria that are causing it, a urine culture is performed. If your kid has any of the following symptoms, the doctor may prescribe a urine culture.

  • When urinating, the patient complains of a painful sensation
  • He or she has the need to pee frequently but does not produce much urine (this is referred to as urgency)
  • Has a fever that does not have a clear cause or is experiencing stomach pain when they have an abnormal regular urinalysis, especially if they have a high amount of white blood cells in their urine when you have finished therapy for a UTI, you should check to see if the infection is gone

Preparation

There is no preparation necessary for the urine culture other than cleaning the region surrounding the urinary entrance before taking the sample. Inform your doctor if your kid is currently receiving antibiotics or has recently taken antibiotics.

The Procedure

The sample collection process should just take a few minutes. In the doctor’s office, your child will be asked to pee into a sterile sample cup, which will be disposed of afterward. It may be necessary to insert a catheter (a narrow soft tube) into your child’s bladder in order to obtain the urine specimen if he or she has not been potty trained and cannot pee into a cup. Just before the urine is collected, it is necessary to clean the skin around the urinary entrance to prevent infection. In this “clean-catch” procedure, you or your kid cleans the skin surrounding the urinary hole with a special towelette.

The idea is to catch the pee in the “middle of the stream.” The container should not come into contact with your child’s skin.

It’s sometimes ideal to take a sample first thing in the morning when your child has woken up, rather than later.

If this is the case, you may be requested to assist your kid in completing the exam at his or her residence. You’ll take the sample to a lab, where a technician will examine it for the presence of germs, and then you’ll return home. Follow any storage and shipping instructions the lab sends you.

What to Expect

Because the test includes typical urination, your kid should not experience any discomfort as long as he or she is able to deliver a sample of urine. (If a catheter was used to collect urine, there may be some pain for a short period of time.) Preparation for the urine test should include keeping the region surrounding the urinary entrance clean and catching the urine sample in the middle of the stream.

Getting the Results

It will take 1-3 days for the results of the urine culture to be obtained. It is your doctor’s responsibility to go through the results with you and explain what they signify.

Risks

When submitting a urine sample for a urine culture, there are no hazards associated. If a catheterized specimen is necessary, there may be some pain for a brief period. Questions concerning this operation can be discussed with your healthcare professional at any point during the process.

Helping Your Child

Urinating to produce the samples for the test is typically a painless experience for the patient. Explain to your kid how the test will be administered and why it is being done in order to allay his or her anxieties. Make sure your youngster understands that the urinary entrance must be clean and that the pee must be collected in the middle of the urine stream to prevent infection.

If You Have Questions

If you have any queries concerning the urine culture, you should consult with your physician.

Urine Culture: Purpose, Results & What To Expect

A urine culture test examines a sample of urine for the presence of germs (microorganisms) that might cause illness. Urine is the liquid waste produced by your body (pee). Growing microorganisms such as bacteria and yeast in a laboratory setting is referred to as culture in medical terminology. A urine sample is supplemented with growth-promoting chemicals in a laboratory. If bacteria or yeast (a fungus) are present, they begin to reproduce and spread. An infection in your urinary system is indicated by the presence of this growth.

What is the purpose of a urine culture?

Urine cultures are ordered by healthcare professionals to screen for urinary tract infections (UTIs). A urinary tract infection (UTI) can arise when bacteria enter your urethra, which is the tube that transports urine from your body. UTIs are most commonly caused by bacteria in your bladder (the organ that holds urine). They have the potential to spread to your kidneys (the organs responsible for the production of urine) or your prostate. A urine culture test will also reveal the following:

  • The bacteria or yeast that is causing the illness must be identified before your healthcare practitioner can choose the most appropriate therapy for you. It is also necessary to know whether the bacterium is resistant to antibiotics.

Who needs a urine culture?

If you have recurrent or difficult-to-treat urinary tract infections, your healthcare professional may recommend a urine culture test. In most cases, only those who are experiencing symptoms of a UTI require a urine culture. UTIs can affect people of either gender, although women are more likely than males to experience them. The following are risk factors for recurrent UTIs:

  • Having diabetes
  • Having frequent sexual relations, especially with new partners or if you use spermicides
  • Having diabetes Kidney disease, particularly kidney stones, is a common occurrence. Problems with completely emptying your bladder, particularly if you use a urinary catheter to drain urine
  • Due to autoimmune illnesses, organ transplantation, or cancer treatment procedures, the immune system has been weakened.

What is the difference between a urine culture test and urinalysis?

A urine sample is required for both a urinalysis and a urine culture. Aurinalysis may be performed first by your healthcare professional. In a shorter amount of time, this test checks urine for the presence of red and white blood cells, as well as bacteria, which can indicate the existence of an illness.

A urinalysis will not be able to identify the precise bacterium that is causing the UTI. A urine culture is required to obtain this information.

Can a urine culture detect a sexually transmitted disease (STD)?

Historically, healthcare practitioners employed bacterial culture tests to identify sexually transmitted diseases (STDs) such as chlamydia and gonorrhea. This was not a urine culture test, as the name implies. Instead, healthcare practitioners cultivated (cultured) cells from within the urethra to test their effectiveness. The indications of these STDs may now be detected by the use of a urine test. However, when it comes to diagnosing an STD, healthcare experts tend to rely on more precise measures such as analyzing fluid from the vaginal or penis.

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Can a urine culture detectE. coli?

A urine culture test can be used to identify the bacterium Escherichia coli (E. coli). The bacteria E. coli is the most common cause of urinary tract infections. E. colibacteria are bacteria that dwell in the digestive system and are discovered in human feces. If fecal matter makes its way from your anus to your vulva or penis, germs can enter your urethra and cause a urinary tract infection (UTI). Close to your anus is your vulva (the outside portion of your female genitals, which is where your vagina and urethra are located).

Everyone, regardless of gender, should wipe their hands from front to back after using the bathroom in order to avoid this type of illness.

Can a urine culture detectStreptococcus(strep) infections?

Group B strep bacteria are a less prevalent cause of urinary tract infections (UTIs). These bacteria, which can be found in the urinary and digestive systems, can be detected using a urine culture. Group B strep is more likely to cause urinary tract infections (UTIs) in pregnant women. It is essential to treat the infection with antibiotics before to delivery. Preventing the transmission of microorganisms to a baby through treatment is important for pregnant women. Antibiotics are also required in the case of a strep B infection in a baby.

Test Details

Before delivering a urine sample, your healthcare professional will inform you whether or not you need to take any particular precautions. Your healthcare practitioner may ask you to do one or more of the following:

  • Please refrain from peeing for at least an hour before providing a urine sample. Ensure that you drink at least 8 ounces of water 20 minutes before the sample collection to ensure that there is enough urine for testing. First thing in the morning, collect a sample of your pee.

What happens during a urine culture?

A clean catch pee sample is required for a successful urine culture. In this context, a urine sample that is as devoid of external pollutants as possible, such as natural bacteria that dwell on your skin, is meant. You may choose to submit this sample in person at your healthcare provider’s office or at a laboratory testing center. In some cases, you may be able to collect the urine sample at your residence. The following are the steps:

  1. Hands should be washed with soap and warm water. To completely clean the opening of the urethra (including the vulva and vaginal region, as well as the head of the penis), use an antiseptic wipe. Allow a tiny bit of pee to flow into the toilet and then stop in the middle of it
  2. Remove the cup from beneath the vulva or penis and replace it before you continue peeing. Do not allow the cup to come into contact with your skin. Fill the cup halfway with pee to the required amount (usually 1 to 2 ounces). The majority of people finish peeing before they complete filling the cup. Stop urinating in the middle of the stream once more (if feasible) and keep the cup out of the way until you’re finished
  3. Deposit the cup on the ground, cover it with a lid (if one is supplied), and place it in the appropriate collecting location. Remember to wash your hands one more before continuing.

What are other ways to collect a urine sample?

A healthcare professional may utilize one of the following ways to treat newborns and young children, as well as people who are unwell, hospitalized, or elderly:

  • It is necessary to catheterize your bladder in order to reach it. Your healthcare practitioner will place a catheter (a thin, flexible tube) via your urethra. A sterile collecting bag is placed over the catheter to catch the urine as it exits. When you have an aspiration, your healthcare professional inserts a small needle into your bladder through numbed abdomen skin in order to pull urine into a collecting bag. Urine collection bag (U bag): For babies and young children, you may want to place a urine collection bag on their penis or to their vulva with sticky glue to prevent them from peeing in their diaper. After your kid has urinated, you should dump their pee into a container with a lid. Preserve the container in the refrigerator until you deliver it to the office or lab of your healthcare practitioner.

How long does a urine culture take?

The process of providing a clean urine sample takes only a few minutes. Making a poop in the cup shouldn’t take long at all. Do take the time to clean your vulva or penis before you pee in order to guarantee a clean urine sample is collected during the test.

After receiving your urine sample, the lab will grow the culture in an incubator for 24 to 48 hours before testing it. The temperature of the incubator is set at 98.6 degrees Fahrenheit, which is the typical temperature for the human body (37 degrees Celsius).

What are the risks of a urine culture?

The clean catch method is a fairly safe way to collect a urine sample from a patient. When using the catheter or needle approach, there is a small chance of contracting an infection.

Results and Follow-Up

In certain cases, the lab may take up to three days to perform the test and deliver the findings back to you. A phone call or an appointment will be scheduled with your healthcare professional in order to go through the results.

What does a positive urine culture test result mean?

If bacteria develop in the urine culture test and you are experiencing symptoms of infection or bladder discomfort, you most likely have a urinary tract infection (UTI). An abnormal test result or a positive urine culture test result are both possible outcomes. The bacteria in the cultivated sample are subjected to an antibiotic sensitivity test in the laboratory. This test, which is also known as an antibiotic susceptibility test, determines the type of bacteria that is causing the illness as well as which antibiotics the bacterium is susceptible to, which means which medications will kill or inhibit the bacteria.

Certain antibiotics are effective exclusively against specific microorganisms.

This signifies that the antibiotic is no longer effective at preventing the growth of that particular species of bacteria.

What does a negative urine culture test result mean?

It is possible to have a positive, or normal, urine culture test result if the urine sample did not contain any bacteria or yeast. You do not have a urinary tract infection. A lab’s typical test results range might vary based on the lab that is doing the test. If you continue to have symptoms such as painful urination (dysuria) or blood in the urine (hematuria), your healthcare practitioner may recommend imaging scans or other testing. These symptoms may be indicative of bladder cancer in certain cases, however this is extremely unusual.

What should I ask my healthcare provider?

You might wish to inquire with your service provider about the following:

  • What is the purpose of a urine culture test
  • Is it necessary for me to fast (that is, not eat or drink) or to cease smoking or taking medications before the test
  • When will I receive the results of the tests
  • Is it necessary for me to be concerned about the test findings
  • Will I be required to take any extra tests? What can I do to avoid getting a UTI?

An announcement from the Cleveland Clinic A urine culture is performed in order to identify microorganisms that cause UTIs. If you have a urinary tract infection, an antibiotic sensitivity test might help you identify the bacterium that is causing it. This information assists your healthcare professional in selecting the most appropriate medication to treat your infection. Following therapy, a urine culture test may be performed to check that your infection has been eradicated. For the majority of people, a simple clean catch urine sample is all that is required by the lab for the test.

If you have a history of urinary tract infections, speak with your healthcare practitioner about actions you may take to reduce your risk of developing them.

Urine culture

urine for culture and sensitivity testing A urine culture is a laboratory test that is used to determine if a urine sample contains bacteria or other pathogens. It may be used to screen for a urinary tract infection in both adults and children, according to the manufacturer. When doing a clean-catch urine sample, it is necessary to capture a sample of urine while it is still flowing. Men or boys should clean the head of the penis with a clean cloth. Women or girls should cleanse the region between the lips of the vaginal opening with soapy water and thoroughly rinse it.

Catch roughly 1 to 2 ounces of pee in a clean container, and then withdraw the container from the urine stream to avoid contamination.

The female and male urinary systems are nearly identical, with the exception of the length of the urethra, which differs. The male and female urinary systems are nearly identical, with the exception of the length of the urethra, which differs.

How the Test is Performed

A clean catch urine sample will be taken in most cases, either at your health-care provider’s office or at your residence. To collect the urine, you will need to utilize a specific kit. An alternative method of collecting a urine sample is to put a thin rubber tube (catheter) into the urethra and into the bladder. Your provider’s office or the hospital will have someone complete this task for you. The urine is drained into a sterile container, and the catheter is then pulled out of the bladder.

The urine is sent to a laboratory for testing to detect whether or not bacteria or yeast are present in the urine sample.

How to Prepare for the Test

Try to collect the sample after your pee has been sitting in your bladder for 2 to 3 hours, if at all feasible.

How the Test will Feel

The catheter may cause you to feel pressure when it is being inserted. The urethra is numbed with the use of a specific gel.

Why the Test is Performed

If you are experiencing signs of a urinary tract infection or a bladder infection, such as discomfort or burning when peeing, your healthcare professional may recommend this test. After you have been treated for an illness, you may be required to have a urine culture taken. This is done to ensure that all of the bacteria has been eliminated. Generally speaking, going to the restroom is a no-brainer. You go to the bathroom, flush the toilet, and wash your hands. However, it is possible to have a disease that makes going to the bathroom uncomfortable or difficult.

  • UTIs are most commonly caused by bacteria getting into your bladder, kidneys, ureters (the tubes that transfer urine from the kidneys to the bladder), or urethra (the tube that transports pee to the outside of your body) and causing irritation or inflammation.
  • Women are more susceptible to bacteria entering their bodies after having sex or going to the restroom than men.
  • Diabetes is the most common risk factor for urinary tract infections.
  • It is possible that your urine could seem hazy or red, and that it will smell awful.
  • It is possible that further urinary abnormalities will be discovered via the use of other scans such as a CT scan and a kidney scan on occasion.
  • Antibiotics are medications that destroy germs.
  • Antibiotics may usually clear up a urinary tract infection in a matter of days or even hours.
  • It is possible that you will need to continue taking antibiotics for a longer period of time.
  • The use of probiotics, or good bacteria, may also be effective in the prevention of urinary tract infections.
  • Clean your genital region thoroughly after using the bathroom, urinating before and after sexual activity, and wiping your genital area from front to back after using the bathroom.
  • Topical estrogen has been shown to significantly prevent urinary tract infections in postmenopausal women.

Recurrent, uncomplicated urinary tract infections are quite prevalent in young women who are not pregnant and otherwise healthy. Fortunately, they are easily treatable and are unlikely to result in any additional health complications.

Normal Results

“Normal growth” is a perfectly normal outcome. This indicates that there is no infection present. In certain cases, the normal value ranges may change somewhat between various laboratories. Some laboratories employ various methods of measurement or examine different substances. Speak with your doctor about the significance of the specific test findings that you received.

What Abnormal Results Mean

When bacteria or yeast are identified in the culture, the test is either “positive” or “abnormal.” This indicates that you have a urinary tract infection or a bladder infection, which is likely the case. Other tests may be performed to assist your physician in determining which bacteria or yeast is causing the illness and which antibiotics will be most effective in treating it. It is possible to find more than one species of bacteria in a culture, or simply a little number of bacteria in a culture.

Risks

If your provider uses a catheter, there is a very small chance that a hole (perforation) will develop in the urethra or bladder.

Considerations

If you have been taking antibiotics, it is possible that you will have a false-negative urine culture.

References

Cooper KL, Badalato GM, Rutman MP; Badalato GM, Rutman MP. Urinary tract infections are infections of the urinary tract. The following chapter is from the 12th edition of Campbell-Walsh-Wein Urology: Chap 55. Partin AW, Dmochowski RR, Kavoussi LR, Peters CA, eds. Campbell-Walsh-Wein Urology, 12th edition, Philadelphia, PA: Elsevier; 2021:chap 55. Nicolle LE, Drekonja D. Nicolle LE, Drekonja D. Patient with urinary tract infection is addressed in the following way: Goldman L, Schafer AI, eds. Goldman-Cecil Medicine.

Chap 268 in the Elsevier 26th edition, Philadelphia, PA, 2020.

Version Info

The most recent review was performed on 10/10/2020. Linda J. Vorvick, MD, Clinical Associate Professor, Department of Family Medicine, University of Washington Medicine, School of Medicine, University of Washington, Seattle, WA, provided the review for this article. In addition, David Zieve, MD, MHA, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial staff examined the manuscript for accuracy.

Urine Culture – Clean Catch

A urine culture is a laboratory test that is used to determine if a urine sample contains bacteria or other pathogens. It may be used to screen for a urinary tract infection in both adults and children, according to the manufacturer.

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Alternative Names

urine for culture and sensitivity testing

How the Test is Performed

A clean catch urine sample will be taken in the majority of cases, either at your health care provider’s office or at your residence. To collect the urine, you will need to utilize a specific kit. An alternative method of collecting a urine sample is to put a thin rubber tube (catheter) into the urethra and into the bladder. Your provider’s office or the hospital will have someone complete this task for you. The urine is drained into a sterile container, and the catheter is then pulled out of the bladder.

The urine is sent to a laboratory for testing to detect whether or not bacteria or yeast are present in the urine sample. This takes between 24 and 48 hours.

How to Prepare for the Test

Try to collect the sample after your pee has been sitting in your bladder for 2 to 3 hours, if at all feasible.

How the Test will Feel

The catheter may cause you to feel pressure when it is being inserted. The urethra is numbed with the use of a specific gel.

Why the Test is Performed

If you are experiencing signs of a urinary tract infection or bladder infection, such as discomfort or burning when peeing, your healthcare professional may recommend this test. After you have been treated for an illness, you may be required to have a urine culture taken. This is done to ensure that all of the bacteria has been eliminated.

Normal Results

“Normal growth” is a perfectly normal outcome. This indicates that there is no infection present. In certain cases, the normal value ranges may change somewhat between various laboratories. Some laboratories employ various methods of measurement or examine different substances. Speak with your doctor about the significance of the specific test findings that you received.

What Abnormal Results Mean

When bacteria or yeast are identified in the culture, the test is either “positive” or “abnormal.” This indicates that you have a urinary tract infection or a bladder infection, which is likely the case. Other tests may be performed to assist your physician in determining which bacteria or yeast is causing the illness and which antibiotics will be most effective in treating it. It is possible to find more than one species of bacteria in a culture, or simply a little number of bacteria in a culture.

Risks

There is an extremely uncommon chance of a hole (perforation) in the urethra or bladder if your physician uses a catheter.

Considerations

If you have been taking antibiotics, it is possible that you will have a false-negative urine culture.

References

Dean, A.J., and Lee, D.C. Microbiologic and analytical procedures performed at the bedside. Roberts and Hedges’ Clinical Procedures in Emergency Medicine and Acute Care is an edited collection by Roberts JR, Custalow CB, and Thomsen TW (eds.). 7th edition, Philadelphia, PA: Elsevier Science; chapter 67. Germann CA, Holmes JA. Germann CA, Holmes JA Selected urologic diseases are listed below. Emergency medicine concepts and clinical practice are covered in Rosen’s Emergency Medicine: Concepts and Clinical Practice.

The ninth edition, Philadelphia, PA: Elsevier; chapter 89.

Campbell-Walsh Urology, edited by Wein AJ, Kavoussi LR, Partin AW, and Peters CA, is available online.

Urinalysis and Urine Culture – Kidney and Urinary Tract Disorders

Urinalysis is a procedure that requires

  • The use of chemical tests to identify and evaluate the levels of different compounds in the urine is becoming more common. Frequently, urine is examined under a microscope

Protein, glucose (sugar), ketones, blood, and other compounds are tested for using chemical methods. A tiny strip of plastic (dipstick) is used in these tests, which is impregnated with chemicals that react with compounds in the urine and cause the color to change fast. Occasionally, the results of the urine test are validated by a more sophisticated and accurate laboratory study of the urine specimen. The presence of red and white blood cells, crystals, and casts in the urine can be determined by examining it under a microscope (impressions of the kidney tubules created when urinary cells, protein, or both precipitate out in the tubules and are passed in the urine).

  • Depending on the reason, protein may be present in the urine on a consistent basis or just infrequently.
  • Small quantities of protein in the urine may be a warning indication of kidney damage caused by diabetes in the early stages of disease.
  • During these instances, urine must be collected over a period of 12 or 24 hours and examined in a laboratory.
  • As a result of the breakdown of fat, ketones are produced.
  • Blood in the urine (hematuria) can be detected using a dipstick and verified with further tests such as inspecting the urine with a microscope and other procedures.
  • A dipstick can be used to identify the presence of leukocyte esterase (an enzyme present in some white blood cells) in the urine.
  • A dipstick is used to determine the acidity (pH) of urine.

Some people are predisposed to kidney stones as a result of a change in their acidity.

The concentration of urine in the bloodstream can also be useful in identifying faulty kidney function.

One unique test requires a person to refrain from drinking any water or other fluids for 12 to 14 hours.

Following that, the concentration of urine is determined.

When it comes to specific kidney illnesses (such as nephrogenic diabetes insipidus), this is not the case.

In nephrogenic diabetes insipidus, the kidneys generate a high volume of dilute urine because the renal tubules fail to react to vasopressin (antidiuretic hormone) and are unable to reabsorb.

Sedimentin urine may be inspected under a microscope to see if a kidney or urinary tract problem is present, providing valuable information.

Someone suffering from renal or urinary tract disease tends to excrete more cells, which can accumulate in the urine when it is centrifuged or allowed to settle. If the urine is centrifuged or allowed to settle, the cells can form a sediment that can be collected and analyzed later.

Urinalysis – Mayo Clinic

A urinalysis is a test that is performed on your pee. Urinary tract infections, renal illness, and diabetes are just a few of the conditions for which it is used to diagnose and treat patients. A urinalysis is a procedure that involves examining the appearance, concentration, and content of urine samples. An infection of the urinary system, for example, might cause urine to appear hazy rather than clear. In certain cases, increased amounts of protein in the urine might indicate the presence of renal disease.

Why it’s done

A urinalysis is a frequent test that is performed for a variety of reasons, including:

  • In order to assess your general health. It is possible that a urinalysis will be performed as part of a standard medical exam, pregnancy checkup, or pre-surgical preparation. When you’re admitted to a hospital, it may be used to screen for a number of problems, such as diabetes, renal disease, or liver disease
  • Or it might be used to screen for a variety of disorders, such as cancer. In order to determine the existence of a medical problem. If you are experiencing stomach discomfort, back pain, frequent or painful urination, blood in your urine, or any other urinary issues, your doctor may recommend a urinalysis. A urinalysis can aid in the identification of the underlying cause of these signs and symptoms. In order to keep track of a medical condition. After being diagnosed with a medical problem, such as kidney disease or a urinary tract infection, your doctor may recommend testing your urine on a regular basis to keep track of your progress and treatment.

A urine sample may also be used for other tests such as pregnancy testing and drug screening, although these procedures check for compounds that aren’t detected by a standard urine analysis.

How you prepare

If you’re merely having a urinalysis, you can eat and drink as much as you like before the test. In the event that you are scheduled for further exams, you may be required to fast before the test. Specific instructions will be provided to you by your health-care professional. Many medicines, including over-the-counter pharmaceuticals and dietary supplements, might have an impact on the findings of a urinalysis. Inform your doctor about any drugs, vitamins, or other supplements you are taking before having a urinalysis performed.

What you can expect

You may choose to collect a urine sample at home or at the office of your health-care provider. Typically, providers hand out containers for collecting urine samples. It is possible that you may be requested to collect the sample at your residence first thing in the morning, when your pee will be at its most concentrated. It is possible that you will be required to collect the sample in the middle of the process, utilizing a clean-catch approach. The following are the stages involved in this method:

  • Cleanse the entry of the urine bladder. Women should spread their labia and clean from the front to the back of their bodies. Men should clean the tip of their penis with a damp cloth. start using the toilet and urinating into it Pass the collecting container into your urine stream
  • This will help to prevent infection. Fill the collection container with at least 1 to 2 ounces (30 to 60 milliliters) of urine
  • Complete your urination in the toilet
  • Deliver the sample in accordance with the instructions provided by your health care practitioner. Refrigerate the sample if it cannot be delivered to the specified place within 60 minutes after its collection, unless your provider has instructed otherwise.

It may be necessary to put a thin, flexible tube (catheter) through the entrance of the urinary tract into the bladder in order to collect a urine sample in some instances. The urine sample is taken and forwarded to a laboratory for testing. You are free to resume to your normal activities right away.

Results

An examination of your urine sample using three methods is performed during a urinalysis. These methods are: visual examination; dipstick examination; and microscopic examination.

Visual exam

The look of the urine is examined by a lab professional. Urine is usually clear in color. The presence of cloudiness or an odd odor may suggest the presence of a condition such as an infection. The presence of protein in urine might cause it to appear frothy. The presence of blood in the urine might cause it to appear red or brown. Urine color might be affected by what you’ve eaten recently as well as by some medications you’re taking. Beets and rhubarb, for example, may cause your urine to become crimson.

Dipstick test

A dipstick — a small, plastic stick with chemical strips attached to it — is inserted into the urine sample.

The color of the chemical strips changes if specific compounds are present or if their concentrations are higher than normal limits. A dipstick test looks for the following things:

  • Acidity is a term used to describe the state of being acidic (pH). In urine, the pH level shows the quantity of acid present in the urine. In some cases, a high pH level might suggest a kidney or urinary tract disease
  • Concentration The concentration of the particles in your urine may be determined by measuring the concentration of the particles. A greater than usual concentration is frequently caused by a lack of fluid intake
  • Protein intake is also inadequate. Protein concentrations in urine are typically low. In most cases, small increases in protein in urine are not a cause for worry, but bigger quantities may signal a kidney disease
  • Sugar. Most of the time, the amount of sugar (glucose) present in urine is insufficient to be identified. Following the finding of sugar on this test, it is common for follow-up testing for diabetes and ketones to be performed. Ketones found in your urine, like sugar, might be an indication of diabetes and necessitate more testing
  • Bilirubin is one such test. Bilirubin is a byproduct of the destruction of red blood cells. Normally, bilirubin is transported via the bloodstream and into the liver, where it is excreted and absorbed as part of the bile. The presence of bilirubin in your urine might suggest liver damage or illness
  • It could also be an indication of an infection. In your urine, the presence of nitrites or leukocyte esterase – a product of white blood cells — may suggest a urinary tract infection
  • Blood may be present. Additional testing is required if you have blood in your urine. Symptoms of kidney damage or infection, kidney or bladder stones, kidney or bladder cancer, and blood abnormalities can all be seen with this condition.

Microscopic exam

This test, which is sometimes done as part of a urinalysis, involves looking via a microscope at droplets of concentrated pee — urine that has been spun in a machine — to look for signs of infection. If any of the following levels are much higher than typical, you may require further testing:

  • The presence of white blood cells (leukocytes) may indicate the presence of an infection. If you have high levels of red blood cells (erythrocytes), it might indicate that you have renal illness. It could also indicate that you have a blood issue or some underlying medical condition such as bladder cancer. Infection is indicated by the presence of bacteria, yeast, or parasites. Casts, which are tube-shaped proteins, can develop as a result of renal disease. In some cases, crystals formed by chemicals in urine may be an indication of kidney stones.

A urinalysis by alone does not generally yield a definitive diagnosis in most cases. Depending on the reason your physician ordered this test, you may require follow-up if the findings are out of the ordinary. The findings of the urinalysis, in conjunction with the results of other tests, can assist your provider in determining the next steps. You should never assume that you are not sick just because you have normal test results from your urinalysis. It is possible that the sickness is detected too early, or that your urine is overly diluted.

Consult with your health-care practitioner for further information on the significance of your urinalysis findings.

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